Fig. 3

Experimental design showing creation of the murine model of early life vitamin D deficiency. All mice underwent 2 episodes of mechanical loading of their left tibia at age 8 and 16 weeks. The right tibia was not mechanically loaded and provided an internal control. Following the second episode of loading at 16 weeks of age, mice were culled (aged 18 weeks), and both tibias stored frozen until 3-point bend testing and subsequent DNA extraction from each of the whole bones (n = 6 mice/n = 12 bones per dietary group). Adapted from Borg et al. [24].