Fig. 2

Overexpression of miR-205-5p attenuates fibrosis in atrial muscle cells. HL-1 cells were with 200 μM PA for 24 h for establishing a cell model of atrial fibrosis (recorded as the PA group). A TGF-β1 expression in cells was determined by RT-qPCR. B The secretion of inflammatory factors TNF-α and IL-1β in cells was measured by ELISA. C Expression of fibrosis-related proteins α-SMA, Col I and COL III in cells was determined by western blot analysis. D–F. miR-205-5p and EHMT2 levels in cells were determined by RT-qPCR and western blot analysis. Transfection of miR-205-5p-mimic and mimic-NC in HL-1 cells. G Expression of miR-205-5p in the transfected cells was determined using RT-qPCR. H. TGF-β1 expression in transfected cells was determined by RT-qPCR. I. The secretion of cell inflammatory factors TNF-α and IL-1β in transfected cells was measured by ELISA. J Expression of fibrosis-related proteins α-SMA, Col I, and COL III in transfected cells was determined by western blot analysis. *p < 0.05, vs. the control group or the mimic-NC group