Fig. 3

miR-205-5p targets EHMT2. A The Starbase database predicted the binding site of miR-205-5p and EHMT2. B, C. Luciferase activity and RIP assays were carried out to detect the binding relationship of miR-205-5p and EHMT2. D-E. EHMT2 expression in cells transfected with miR-205-5p-mimic was determined by RT-qPCR and western blot analysis. F, G Expression of EHMT2 in cells co-transfected with miR-205-5p-mimic and oe-EHMT2 was determined using RT-qPCR and western blot. H TGF-β1 expression in cells co-transfected with miR-205-5p-mimic and oe-EHMT2 was determined by RT-qPCR. I The secretion of cell inflammatory factors TNF-α and IL-1β in cells co-transfected with miR-205-5p-mimic and oe-EHMT2 was measured by ELISA. J Expression of fibrosis-related proteins α-SMA, Col I, and COL III in cells co-transfected with miR-205-5p-mimic and oe-EHMT2 was determined by western blot analysis. *p < 0.05, vs. the IgG group, the mimic-NC group or the miR-205-5p-mimic + oe-NC group