Fig. 6
AA supplementation improves eWAT development in GLUT10G128E mice. Mice were treated as described in Fig. 1A. Data were collected from pups at 3 weeks of age. A and B AA supplementation increases percentage of eWAT weight of GLUT10G128E mice. A Body weight and B eWAT percent of total body weight were measured. C–E AA supplementation increases adipocytes size in eWAT of GLUT10G128E mice. C Representative photographs of hematoxylin and eosin (H&E) staining of eWAT sections. The cross-sectional area of adipose cells in eWAT is presented as D average size of cells and E the percentage of cells in the given size range. In D and E, n = 6 mice per group; more than 1000 adipocytes were analyzed in each mouse. F AA supplementation reduces Pref-1 protein levels in eWAT of GLUT10G128E mice. The protein levels of Pref-1 and PPARγ 1 in eWAT were analyzed by Western blotting. Protein samples from 6 mice per group were pooled. The protein levels were quantified, normalized to β-actin levels, and compared to WT no-AA-treated controls. The data are shown as mean ± SEM from triple repeats of Western blotting experiments of the pooled samples. Statistical significance was determined by one-way ANOVA followed by Tukey’s test for multiple comparisons. The compact letter display indicates significant differences in pairwise comparisons; groups with different letters are significantly different