Altered macronutrient composition and genetics influence the complex transcriptional network associated with adiposity in the Collaborative Cross

Table 4 DEGs with expression correlated with body fat % associated with obesity in humans

Module colors	Diet DEGs	Gene DE by diet and strain	Strain DEGs
Turquoise	0	9	106
Yellow	0	19	35
Red	0	0	22
Brown	0	1	15
Green	0	1	15
Black	1	2	13
Blue	1	2	8
Pink	1	6	7
Purple	0	0	6
Salmon	0	0	5
Tan	0	3	5
Greenyellow	0	1	1
Magenta	0	0	1

By intersecting lists of genes across multiple analyses, 434 DEGs in the CC were found to have gene expression levels significantly correlated with body fat % and genes associated with obesity traits in humans in the GWAS catalog, with 3 diet DEGs, 369 strain DEGs, and 62 genes differentially expressed by both diet and strain. The number of genes belonging to each category and assigned to the respective module is shown above, with 148 genes not assigned to any module

ISSN: 1865-3499