Fig. 2
The effect of trace minerals on iron and hypoxia-regulated gene mRNA expression levels in polarized Caco-2 cells under the DFO treatment condition. Caco-2 cells were grown on the bicameral cell culture system for 21 days. Iron deficiency was induced by 200 μM for 24h, and then, the copper (CuCl2; 100 μM) and iron (FAC; 100 μg/mL) were given to apical and basolateral sides of the cell culture system for 18h. The effects of Cu and Cu with Fe on iron (a) and hypoxia (b) regulating gene mRNA expression were analyzed