Table 1 Forward and reverse primers of 5 genes (1) Housekeeping gene GAPDH, (2,3) SDH subunit C, D. (4) Sirtuin-3 (5) HIF
Gene | Forward primer (/5 ––– /3) | Reverse primer (/5 ––– /3) | Size (bp) |
|---|---|---|---|
GAPDH | GATTCCACCCATGGCAAATTC | CTGGAAGATGGTGATGGGATT | 87 |
SDHC | GATGGAGCGGTTCTGGAATAA | CATGGGAAGAGACCAACTGTAG | 85 |
SDHD | CATTTCTTCAGGACCGACCTATC | AACTTGTCCAAGGCCCAAT | 88 |
Sirtuin3 | AGGGACGATGATGTAGCTGA | GGCGATCTGAAGTCTGGAATG | 110 |
HIF | GTCTGCAACATGGAAGGTATTG | GCAGGTCATAGGTGGTTTCT | 103 |