Fig. 3
Contribution of serine one-carbon units for de novo dTMP synthesis. L-[2,3,4-2H3]-serine contribution to dTMP synthesis from formate was reduced with homozygous loss of SHMT2 and in HAP1 cells cultured in low-folate medium compared to HAP1 cells culture in folate-sufficient medium. One-way ANOVA with Tukey’s post hoc analysis was used with a statistical significance of p < 0.05. Data represents means ± SD values, n = 2–3 per group. L-[2,3,4-2H3]-serine incorporation into thymidine is conveyed by the ratio of carbons containing one deuterium atom/total number of carbons containing one and two deuterium atoms