Fig. 6

Cell type ATP production and extracellular acidification rates. Decreased SHMT2 and exposure to low-folate medium impair ATP production and ECAR. ATP production and extracellular acidification rates in A HAP1 cells and ΔSHMT2 cells and B Shmt2^+/+ and Shmt2^+/− MEF cells. ATP production and extracellular acidification rates were normalized to total cell count. Two-way ANOVA with Tukey’s post hoc analysis was used to determine media by genotype interaction and main effects of media and genotype with a statistical significance at p < 0.05. Levels not connected by the same letter are significantly different. Data represent means ± SD values, n = 4 per group with 2 embryo cells lines represented in each group