Fig. 6
Expression of rat and mouse Mptx mRNA and absence of human MPtx expression, using RT-PCR. Lane (L)2: rat cDNA; L3: rat CDNA without reverse transcriptase (RT) step; L4 mouse cDNA; L5 mouse cDNA without RT step; lanes L6–L10: human cDNA from ileum (L6), colon transversum (L7, L9) and rectum (L8); cloned human chromosomal DNA containing Mptx (positive control for PCR) (L10). PCRs were performed with primers for rat Mptx (L2, L3; calculated 220 bp), mouse Mptx (L4, L5; calculated 145 bp), human Mptx (L6-L9; calculated 484 bp) and, to control human cDNA quality, human ß2-microglobulin (L11, re-amplification of L7; calculated 249 bp), as described in the Materials and methods section. L1, L12: molecular weight marker (100bp ladder)