Fig. 1

Changes in the expression of genes encoding mediators of lipid transport in Caco-2 cells treated with 80-μM trans-10, cis-12 CLA relative to 80-μM linoleic acid-treated cells. Up arrow and down arrow indicate up or down-regulation of transcription, fold-change (as determined by microarray analysis) is indicated in brackets and NC no change. Processes represented include: 1 absorption of unesterified cholesterol (UC) mediated by the Niemann–Pick C1-like 1 (NPC1L1) protein, 2 expulsion of cholesterol (absorbed or synthesized locally in the enterocytes) into the intestinal lumen through the actions of the ATP-binding cassette (ABC), subfamily G, member 5/ABCG8 heterodimeric transporter, 3 esterification of cholesterol (CE) by acyl-coenzyme A: cholesterol acyltransferase 2 (ACAT2) for incorporation into chylomicrons (CM), 4 cholesterol transfer to plasma high density lipoproteins (HDL) mediated by ABCA1, 5 fatty acids (FA) absorption and transport of FA by fatty acid binding proteins (FABP)-1 and FABP-6 to the endoplasmic reticulum for incorporation into triglyceride (TAG) and phospholipids, 6 TAG synthesis pathways: rate-limiting enzymes in the monoacylglycerol and glycerol 3-phosphate pathways, respectively, are diacylglycerol acyltransferase (DGAT) and glycerol-3-phosphate acyltransferase (GPAT), 7 chylomicron assembly and secretion, 8 transfer of proteins and lipid subfractions between CM and plasma lipoproteins (i.e. HDL). Apolipoprotein (apo), apoB mRNA editing enzyme (APOBEC1), microsomal triglyceride transfer protein (MTP)