Fig. 1

Changes in expression of typical for cardiomyogenesis genes in human SVF cells cultured in presence or absence of selected cytokines. 24-day incubation of freshly isolated human SVF cells in Methocult medium supplemented with SCF (50 ng/ml), IL-3 (10 ng/ml) and IL-6 (10 ng/ml) was followed by total RNA isolation and RT-PCR analysis of ANP, GATA4, MEF2C and MYOD1 gene expression. The SVF cells cultured in adaptation medium were used as controls. Results are presented as average ± SEM from three separate experiments. Statistical significance was set at P < 0.05. SCF Stem cell factor, ANP atrial natriuretic peptide, GATA4 GATA binding protein 4, MEF2C myocyte enhancer factor 2C, MYOD1 myogenic differentiation 1