Table 1 Sequences of PCR primers used
From: Effects of the Sri Lankan medicinal plant, Salacia reticulata, in rheumatoid arthritis
Target gene | Sequence | Target gene | Sequence |
|---|---|---|---|
GAPDH forward | 5′-TTGACCTCAACTACATGG-3′ | MMP-2 forward | 5′-CAGTGACACCACGTGACAAG-3′ |
GAPDH reverse | 5′-CAGGGTGGTGGACCTCAT-3′ | MMP-2 reverse | 5′-CATTCCAGGAGTCTGCGATG-3′ |
TNF-α forward | 5′-CGCTCTTCTGTCTACTGAAC-3′ | MMP-3 forward | 5′-CCAGTCTACAAGTCCTCCAC-3′ |
TNF-α reverse | 5′-GTCCCTTGAAGAGAACCTGG-3′ | MMP-3 reverse | 5′-GCAGCATCCATGTTGGATGG-3′ |
IL-1β forward | 5′-GCTGTCCAGATGAGAGCATC-3′ | MMP-9 forward | 5′-CTGCCTGCACCACTAAAG-3′ |
IL-1β reverse | 5′-CTGTCCATTGAGGTGGAGAG-3′ | MMP-9 reverse | 5′-GTACAGGAAGAGTACTGC-3′ |
IL-6 forward | 5′-CAGTTGCCTTCTTGGGACTG-3′ | c-fos forward | 5′-GATACACTCCAAGCGGAGAC-3′ |
IL-6 reverse | 5′-CGTAGTCCAGAAGACCAGAG-3′ | c-fos reverse | 5′-CCAGTCTGCTGCATAGAAGG-3′ |
IL-10 forward | 5′-CAATAACTGCACCCACTTCC-3′ | RANKL forward | 5′-GAGCGCAGATGGATCCTAAC-3′ |
IL-10 reverse | 5′-ATTCATGGCCTTGTAGACAC-3′ | RANKL reverse | 5′-GCAGCATTGATGGTGAGGTG-3′ |
IFN-γ forward | 5′-GATATCTGGAGGAACTGG-3′ | Cathepsin K forward | 5′-CCAGTGTGGTTCCTGTTGG-3′ |
IFN-γ reverse | 5′-GACTCCTTTTCCGCTTCC-3′ | Cathepsin K reverse | 5′-TTGCCGTGGCGTTATACAT-3′ |