Fig. 1

Effect of sugar on the fat accumulation in nematode. a The synchronized L1 larva was bred on the NGM plate containing glucose (0, 0.1, 1, 2, 5, 10, 50 and 100 mM) with E. coli. After 4 days, the fat was dyed with Nile red and observed under a fluorescent microscope. Upper panel: bright field, middle panel: fluorescent field (Nile red), lower panel: fluorescent field (Nile red), magnified. b The synchronized L1 larva was bred on the NGM plate containing various types of sugars (10 mM) with E. coli. After 4 days, the fat was dyed with Nile red and observed under a fluorescent microscope. Upper panel: bright field, middle panel: fluorescent field (Nile red), lower panel: fluorescent field (Nile red), magnified. c, d Fluorescent intensity of Nile red in (a) and (b) was quantified and plotted on graph, respectively. N = 12, error bar indicates standard error