Fig. 2

Effect of glucose on the gene expression. a The synchronized L1 larva was bred on the NGM plate for 3 days. After 100 μl of glucose (0, 10, 100 and 500 mM) was added on the plate, the worms were cultured for 12 or 24 h with E. coli. The quantity of cDNA was corrected by using gpd-1 (glyceraldehyde 3-phosphate dehydrogenase) as an internal standard. PCR (95°C for 5 min; 23–35 cycles of 95°C for 1 min, 52°C for 30 s, 72°C for 1.5 min; 72°C for 7 min) was performed. b The GFP-expression plasmid that carries the promoter region of sbp-1 (the upstream 3,500 ntds region and the part of 1st exon) was constructed. This sbp-1 promoter-GFP plasmid was injected with lin-15 plasmid into the lin-15 mutant worm, and worms were observed under a fluorescence microscope through the developmental process. Bar = 500 μm