Fig. 3

Effect of sbp-1 RNAi on the phenotype of nematode. (A) The synchronized worms were bred on the RNAi plate (control (L4440), sbp-1) for 4 days. Then, the fat was dyed with Nile red and observed under a fluorescent microscope. The upper panel indicates an optical figure, and the lower panel indicates fluorescent figure. (B) (a) The synchronized worms were bred on the RNAi plate (control (L4440), sbp-1) for 4 days, and the number of laid egg per day was counted. N = 12, (b) The adult worms were bred on the RNAi plate (control (L4440), sbp-1) for 24 h, and the number of laid egg per day was counted. N = 9, error bar indicates standard error. (C) (a) The synchronized worms were bred on the RNAi plate (control (L4440), sbp-1) for 6 days. (b) The wild-type adult worm was bred for 1 day without feeding (a state of starvation). (D) The synchronized worms were bred on the RNAi plate (control (L4440), sbp-1) for 4 days. The number of movements per 1 min of the thrashing (a) and the pumping (b) was counted under a microscope. N = 9, error bar indicates standard error