Fat accumulation in Caenorhabditis elegans is mediated by SREBP homolog SBP-1

Genes & Nutrition

Table 1 Primers for RT-PCR

Gene	Forward	Reverse
sbp-1	CATGAATTCATTCGAGGGAGACGTCCC	CATGAATTCCTGATGTGGAGTCATCGC
fat-1	CAGCTCGAGCACGGCTCCGGTCACCGG	CAGCTCGAGTGACTTTTCTGCCATGGG
fat-2	CAGCTCGAGCAATCCCTGAGCACTGC	CAGCTCGAGAGTCTTCCTCAGTAACCC
fat-3	CATGGATCCACAAGAATGCCTCCGGGC	CATGGATCCAATCCATCATCATGAAGC
fat-4	CAGCTCGAGAGAGCAAGAGCATGAGCC	CAGCTCGAGATCCATCCATAAGTCC
fat-5	CAGCTCGAGCGATTATATCTAAACAG	CAGCTCGAGCAGCATCAGTATCCGTCC
fat-6	CATGGATCCACGGCGGCCCAGAGACGC	CATGGATCCCAGTCCACTTGTGATGGC
fat-7	CATGGATCCCTCAATACCTGTTCATGG	CATGGATCCCAGCATCTGTATCAGTCC
elo-2	CATGGATCCCAAGTCCAGCAGCCACGC	CATGGATCCTCGACATCACAAAGGCCC
acs-2	AGTAAGCTTAATCCTCGTATCCCGCCG	AGTAAGCTTCCTGGCTGGCCACGTCGC
gpd-1	ATGTCGAAGGCCAACGTC	GTTTTGTCCAGCACCGCG

PCR was performed using the primers indicated as below under optimal amplification conditions (95°C for 5 min; 23–35 cycles of 95°C for 1 min, 52°C for 30 s, 72°C for 1.5 min; 72°C for 7 min) for each gene. The PCR amplification of each cDNA was performed independently using three samples in triplicate

ISSN: 1865-3499