Fat accumulation in Caenorhabditis elegans is mediated by SREBP homolog SBP-1

Genes & Nutrition

Table 2 Primers for RT-PCR

Gene	Forward	Reverse
sbp-1	GTCGGATCCTCAGTCATGCCTTAGACC	TATGGTACCGACATAGGGACGTCTCCC
fat-1	CATGTCGACACGCAGTACTGAATAGCC	TATGGATCCAACCAGAACATCTCCGCC
fat-2	TATGGATCCTCCAGGTAGTCAGTACG	CAGGGTACCTTGATTGTATCAAGGTCC
fat-4	CATGTCGACTTCCTCCTTACTCAACGG	TATGATCCCTCATGCTCTTGCTCTCG
fat-5	CATGTCGACCTGCCAATCACAACACCG	TATGGATCCGATCTCGTTCAGGTCCGC
fat-6	CATGGATCCTGTCACACAACACTGAGC	TATGGTACCTCCACGGCGAGATATTGC
elo-2	CATGGATCCTGACGACATCTTACATCG	TATGGTACCTCAGTCTGATCCAGACTC
acs-2	CAGGTCGACTTGCATGTCTGTGAGACC	ATAGGTACCGTGCTTGTGAGCACGTGG

PCR was performed using the primers indicated as below under optimal amplification conditions (95°C for 5 min; 25–30 cycles of 95°C for 1 min, 52°C for 30 s, 72°C for 1.5 min; 72°C for 7 min) for each gene

ISSN: 1865-3499