Table 1 Primer sequences for the selected genes used for validation of the microarray data using real-time relative quantitative PCR (qRT–PCR)
Gene name (Genebank ID) | Primer sequences | Product size (bp) |
|---|---|---|
DHFR (NM_000791) | Forward: 5′ CATGGTCTGGATAGTTGGTGGC 3′ Reverse: 5′ GTGTCACTTTCAAAGTCTTGCATG 3′ | 108 |
TYMS (NM_001071) | Forward: 5′ ATCAAGGGATCCACAAATGA 3′ Reverse: 5′ GGTCAACTCCCTGTCCTGAA 3′ | 205 |
LIPC (NM_000236) | Forward: 5′ CAAGTGCCCTTGGACAAAGC 3′ Reverse: 5′ TGACAGCCCTGATTGGTTTCT 3′ | 130 |
CYP24A1 (NM_000782) | Forward: 5′ CTCATGCTAAATACCCAGGTG 3′ Reverse: 5′ TCGCTGGCAAAACGCGATGGG 3′ | 300 |
PLAUR (NM_002659) | Forward: 5′ TGCGGTGCATGCAGTGTAAGAC 3′ Reverse: 5′ TCAAGCCAGTCCGATAGCTCAG 3′ | 183 |
PLCXD1 (NM_018390) | Forward: 5′ ACGAGTACCTGGTCGCCTGTAT 3′ Reverse: 5′ CATAGGAGACGATGACCTGTTGG 3′ | 117 |
SQSTM1 (NM_003900) | Forward: 5′ CCAGTGACGAGGAATTGACAA 3′ Reverse: 5′ CATCGCAGATCACATTGGGG 3′ | 156 |