Fig. 2

CSE does not directly interfere with the gelatinolytic activity of MMP-2. a Cell culture supernatants of primary chondrocytes isolated from 2 patients (PC1 and PC2) were treated and subjected to gelatine zymography as described in Materials and Methods. Lane 1 shows the activity of a recombinant human pro-MMP-2. Lanes 2 and 4 show the supernatants of untreated chondrocytes, whereas lanes 3 and 5 present the supernatants of the chondrocytes treated with 5 μg/ml CSE, respectively. b Recombinant human pro-MMP-2 was subjected to gelatine zymography and developed separately in different developing buffers containing 5 μg/ml CSE, 10 μg/ml CSE or 1 mM EDTA