Fig. 4

CSE inhibits IL1β-mediated MMP transcription in SW1353 cells. Serum-starved SW1353 cells were pretreated with 5, 10, or 20 μg/ml of CSE followed by the addition of 10 ng/ml IL1β. After 6 h of incubation, total RNA was isolated and mRNA levels of a MMP-1, b MMP-2, c MMP-3, d MMP-7, e MMP-8, f MMP-9, and g MMP-13 were quantified using RT-qPCR and expressed as fold change with respect to untreated controls. Mean values and standard deviations are given. *indicates significant differences with respect to cells treated with IL1β only (P < 0.05). ^#indicates significant differences with respect to untreated controls (P < 0.05)