Fig. 5

Regulation of TIMP transcription by IL1β and CSE in SW1353 cells and primary chondrocytes. a–c Primary chondrocytes were isolated from 3 donors (PC1, PC2, PC3) and cultured and analyzed separately. Serum-starved cells were pretreated with 5 μg/ml CSE followed by the addition of 10 ng/ml IL1β. After 6 h of incubation, the mRNA levels of TIMP-1 (a), TIMP-2 (b), and TIMP-3 (c) were quantified using RT-qPCR and expressed as fold change with respect to untreated controls (white bars). Gray bars represent IL1β-treated cells, whereas black bars represent cells pretreated with CSE. d TIMP-1, TIMP-2, and TIMP-3 mRNA levels were quantified in SW1353 cells under exposure to IL1β and CSE as described. Mean values and standard deviations are given. *indicates significant differences with respect to cells treated with IL1β only (P < 0.05). ^#indicates significant differences with respect to untreated controls (P < 0.05)