Table 1 Details on primers for RT-qPCR
Gene | Accession number | Forward primer | Reverse primer | Amplicon size (bp) | Efficiency (%) |
|---|---|---|---|---|---|
MMP1 | NM_002421 | CTGGAATTGGCCACAAAGTT | TCCTGCAGTTGAACCAGCTA | 144 | 93.9 |
MMP2 | NM_004530 | TGACGGTAAGGACGGACTC | ATACTTCACACGGACCACTTG | 124 | 91.3 |
MMP3 | NM_002422 | GGTGTGGAGTTCCTGATGTTG | AGCCTGGAGAATGTGAGTGG | 184 | 99.9 |
MMP7 | NM_002423 | GAGTGCCAGATGTTGCAGAA | TCTCTTTGCCCCACATGTTT | 156 | 106.9 |
MMP8 | NM_002424 | TCTGCAAGGTTATCCCAAGG | ACCTGGCTCCATGAATTGTC | 154 | 95.3 |
MMP9 | NM_004994 | CTGGGCAGATTCCAAACCT | TACACGCGAGTGAAGGTGAG | 170 | 91.9 |
MMP13 | NM_002427 | TCAGGAAACCAGGTCTGGAG | TGACGCGAACAATACGGTTA | 196 | 102.3 |
TIMP1 | NM_003254 | AATTCCGACCTCGTCATCAG | GTTGTGGGACCTGTGGAAGT | 195 | 100.3 |
TIMP2 | NM_003255 | GTAGTGATCAGGGCCAAAGC | GGGGGCCGTGTAGATAAACT | 153 | 101.9 |
TIMP3 | NM_000362 | GTACCGAGGCTTCACCAAGA | ACCTCTCCACGAAGTTGCAC | 164 | 95.9 |