Fig. 3

Flow cytometric analysis of cell death and apoptosis. Cells were treated with the indicated concentrations of hydroxytyrosol (HT) for 24 h and stained with PI (cell viability) and Annexin V (apoptosis). a Percentage of K562 cells that were unaffected, undergoing apoptosis, undergoing cell death or both cell death and apoptosis. b Relative fluorescence values for PI (cell death) in the cells treated with hydroxytyrosol. Data are representative of at least two independent experiments, and error bars indicate standard error of the mean