Table 3 Anaesthesia and tissue collection procedure prior analysis of brain drug concentrations and their mathematical correction as published in Friden et al. (2010)
From: Do polyphenols enter the brain and does it matter? Some theoretical and practical considerations
Step | Procedure |
|---|---|
1 | Anaesthesia with isoflurane |
2 | Collection of blood sample from abdominal aorta (2Â mL) for analysis |
3 | Exsanguination by severing the heart |
4 | Removal of brain, taking of coronal section for analysis |