Fig. 3

Effect of Se depletion on TNFα and flagellin stimulated NF-κB-driven reporter activity. Stably transfected Caco-2 cells were grown for 3 days in either Se-depleted or Se-supplemented (40 nM) medium and then stimulated with a 20 ng/ml TNFα for 4 h or b 100 ng/ml flagellin for 6 h. Luciferase activity was measured in cell extracts, calculated per mg cell protein and expressed relative to the activity in the Se-supplemented NF-κB-luciferase cells. *P < 0.05