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Fig. 7 | Genes & Nutrition

Fig. 7

From: Differential effects of selenium and knock-down of glutathione peroxidases on TNFα and flagellin inflammatory responses in gut epithelial cells

Fig. 7

Selenoprotein mRNA expression in Caco-2 cells after GPX1 siRNA treatment. a Caco-2 cells grown in Se-supplemented medium were treated with 80 pmol GPX1 siRNA or negative control siRNA for 48 h, RNA extracted and selenoprotein mRNA levels assessed by semi-quantitative RTPCR. b After separation of the RTPCR products by gel electrophoresis, the intensity of bands corresponding to the amplified products was measured using UV Band software. Selenoprotein mRNA expression was normalised to GAPDH and calculated relative to the expression in the negative control siRNA group. Expression of GPX1 was significantly lower in GPX1 siRNA treated cells but expression of SELH, SELW, GPX4 and GPX2 was unaffected. **P < 0.01

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Genes & Nutrition

ISSN: 1865-3499

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