Fig. 1

HASMC growth in the presence of C18:1 fatty acid isomers. a HASMC were pre-treated for 24 h in DMEM with 0.5% BSA and then exposed to control medium (ctr), 50 and 100 μM of oleic acid (OA) for 48 h. Protein lysates were prepared for the analysis of scd1 protein expression by Western blotting. b HASMC were pre-treated for 24 h in DMEM with 0.5% BSA and then treated with 100 μM of oleic acid. Cells were collected at various time points for analysis of scd1 protein expression by Western blotting. c HASMC were pre-treated for 24 h in DMEM with 0.5% BSA. At day 0, cells were treated with oleic acid (OA), trans vaccenic acid (VA) and elaidic acid (ELA) at 100 μM. Proliferation control cells were maintained in DMEM with 10% FBS. Cell number was determined by Cyquant^® kit. Values represent the mean ± SEM (n = 3)