Fig. 2
Scd index and delta-9 desaturation of HASMC treated with C18:1 fatty acid isomers. a HASMC were treated with 100 μM of oleic acid (OA), trans-11 vaccenic acid (VA) or trans-9 elaidic acid (ELA) for 48 h and total fatty acid composition was analysed by gas chromatography. Scd index was determined as C16:1n-7/C16:0 or C18:1 n-9/C18:0. Values are the mean ± SEM of three independent experiments. *Significantly different (P < 0.05). b Determination of delta-9 desaturation rate in HASMC exposed to 100 μM of fatty acid compared to control (ctrl) HASMC. HASMC treated with C18:1 isomers for 48 h were incubated with [14C] stearic acid for further 6 h, and total lipid extraction was performed for radiolabelled fatty acid separation by RP-HPLC. Values represent the mean ± SEM of three independent experiments. Bars assigned different superscript letters (a, b, c and d) were significantly different (P < 0.05)