Fig. 4
Regulation of scd1 transcription by C18:1 fatty acids in HASMC. a Analysis of scd1 mRNA expression by RT-qPCR in HASMC treated for 48 h at 100 μM oleic acid (OA), trans vaccenic acid (VA) and trans elaidic acid (ELA) compared to control BSA (ctr). b Quantification of human scd1 promoter luciferase activity normalized with β-galactosidase expression vector activity in HASMC treated with OA, VA and ELA at 100 μM for 48 h compared to control cells (ctr). Values represent the mean ± SEM of three independent experiments. Bars assigned different superscript letters (a, b, c and d) were significantly different (P < 0.05)