Fig. 5

Effect of CLA on Fasn and Scd1 gene expression in C2C12 myotubes under conditions inducing expression of markers of ER stress. Fully differentiated myotubes were incubated at day 9 with a CLA mixture (20 μM) and/or palmitic acid (0.5 mM), in the absence or in the presence of insulin (100 nM) for 24 h in serum-free differentiation media. CLA mixture was an equimolar solution (50:50) of both active isomers trans-10, cis-12 and cis-9, trans-11 CLA. Fasn (a) and Scd1 (b) data are expressed as mean ± SEM of three independent experiments yielding essentially the same results (n = 4–5 in each experiment). Mean values with unlike letters are significantly different (P < 0.05); ANOVA followed by LSD test