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Table 1 Relative changes in the expression of genes encoding transcription factors and co-activators, myogenic markers, and proteins involved in substrate handling and energy metabolism in gastrocnemius muscle of mice treated with CLA and fed a standard-fat diet

From: Transcriptional analysis reveals a high impact of conjugated linoleic acid on stearoyl-Coenzyme A desaturase 1 mRNA expression in mice gastrocnemius muscle

Experiment 1 (standard-fat diet)

 

Control

CLA1

CLA2

Transcriptional co-activators

PGC-1α

1.00 ± 0.11

0.87 ± 0.04

1.11 ± 0.10

PGC-1β

1.00 ± 0.06a

1.21 ± 0.05b

1.24 ± 0.05b

Contractility/myogenic markers

MyHC-b

1.00 ± 0.17

0.98 ± 0.10

0.91 ± 0.08

MyHC-IIa

1.00 ± 0.09

0.98 ± 0.06

1.02 ± 0.06

MyHC-IIb

1.00 ± 0.05

0.96 ± 0.03

0.96 ± 0.04

MyHC-IIx/d

1.00 ± 0.06

1.01 ± 0.04

1.14 ± 0.10

Transcription factors

ERRα

1.00 ± 0.05

0.95 ± 0.05

0.90 ± 0.04

PPARα

1.00 ± 0.10

0.88 ± 0.04

0.90 ± 0.06

PPARδ

1.00 ± 0.03

0.99 ± 0.02

1.07 ± 0.03

Fatty acid metabolism

Cd36

1.00 ± 0.07

0.82 ± 0.04

0.88 ± 0.09

Lpl

1.00 ± 0.06

0.97 ± 0.03

0.98 ± 0.05

Cpt1b

1.00 ± 0.18

1.22 ± 0.09

1.14 ± 0.09

Acox1

1.00 ± 0.04

0.95 ± 0.02

1.05 ± 0.04

MCAD

1.00 ± 0.11

1.01 ± 0.09

1.21 ± 0.07

Fabp4

1.00 ± 0.08

1.02 ± 0.07

1.16 ± 0.07

Glucose and insulin metabolism

Glut4

1.00 ± 0.06

1.11 ± 0.05

1.10 ± 0.04

Pdk4

1.00 ± 0.10

0.79 ± 0.07

0.84 ± 0.10

Hexokinase 2

1.00 ± 0.05

0.94 ± 0.03

1.00 ± 0.04

Insr

1.00 ± 0.06

0.97 ± 0.06

0.95 ± 0.05

Energy expenditure

Ucp3

1.00 ± 0.09

0.86 ± 0.07

1.03 ± 0.10

  1. RNA was isolated from gastrocnemius muscle of animals fed a standard diet and receiving an oral dose of CLA (3 mg/day in CLA1 and 6 mg/day in CLA2) for 37 days. Expression levels of target genes were measured by real-time PCR and normalized by the average of the housekeeping genes β-actin and GAPDH. The results, mean ± SEM of 8–12 mice/group, are expressed as fold induction over control group. Means in a row without a common letter differ, P < 0.05 (ANOVA followed by LSD test)