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Table 2 Relative changes in the expression of genes encoding transcription factors and co-activators, myogenic markers, and proteins involved in substrate handling and energy metabolism in gastrocnemius muscle of mice treated with CLA and fed a high-fat diet

From: Transcriptional analysis reveals a high impact of conjugated linoleic acid on stearoyl-Coenzyme A desaturase 1 mRNA expression in mice gastrocnemius muscle

Experiment 2 (high-fat diet)

 

Control

CLA3

CLA4

Transcriptional co-activators

PGC-1α

1.00 ± 0.05

1.00 ± 0.05

1.05 ± 0.05

PGC-1β

1.00 ± 0.12

1.13 ± 0.10

1.01 ± 0.12

Contractility/myogenic markers

MyHC-b

1.00 ± 0.08

0.95 ± 0.07

0.87 ± 0.08

MyHC-IIa

1.00 ± 0.09

1.07 ± 0.05

1.08 ± 0.07

MyHC-IIb

1.00 ± 0.12

1.26 ± 0.10

1.14 ± 0.05

MyHC-IIx/d

1.00 ± 0.07

0.88 ± 0.03

0.88 ± 0.05

Transcription factors

ERRα

1.00 ± 0.02

0.89 ± 0.06

0.87 ± 0.05

PPARα

1.00 ± 0.08

1.06 ± 0.07

1.05 ± 0.09

PPARδ

1.00 ± 0.04

1.02 ± 0.06

1.04 ± 0.06

Fatty acid metabolism

Cd36

1.00 ± 0.06

1.05 ± 0.07

0.99 ± 0.06

Lpl

1.00 ± 0.10

0.96 ± 0.07

1.03 ± 0.04

Cpt1b

1.00 ± 0.04

0.94 ± 0.03

0.93 ± 0.04

Acox1

1.00 ± 0.05

0.98 ± 0.04

0.96 ± 0.03

MCAD

1.00 ± 0.08

0.96 ± 0.06

1.08 ± 0.04

Fabp4

1.00 ± 0.04a

0.90 ± 0.06a

1.24 ± 0.07b

Glucose and insulin metabolism

Glut4

1.00 ± 0.06

0.90 ± 0.05

0.89 ± 0.03

Pdk4

1.00 ± 0.04

0.89 ± 0.05

0.84 ± 0.06

Hexokinase 2

1.00 ± 0.08

1.05 ± 0.06

1.02 ± 0.03

Insr

1.00 ± 0.05a

0.78 ± 0.06b

0.75 ± 0.03b

Energy expenditure

Ucp3

1.00 ± 0.05

0.90 ± 0.09

0.90 ± 0.05

  1. RNA was isolated from gastrocnemius muscle of animals fed a high-fat diet and receiving an oral dose of CLA (3 mg/day in CLA3 and 6 mg/day in CLA4) for 30 days and double amount since then and for 35 days. Expression levels of target genes were measured by real-time PCR and normalized by the average of the housekeeping genes β-actin and GAPDH. The results, mean ± SEM of 5-8 mice/group, are expressed as fold induction over control group. Means in a row without a common letter differ, P < 0.05 (ANOVA followed by LSD test)