Table 2 Experiments showing influence of α-tocopherol provision on α-tocopherol levels in liver membranal fractions of rats
Subcellular fractions | Treatments | Results | References |
|---|---|---|---|
Mitochondria (inner and outer), microsomes, and lysosomal and nuclear fraction | Depletion versus 100 mg vitamin E (all-rac-α-tocopheryl acetate)/kg diet | α-Tocopherol levels were 16.2-, 8.3-, and 2.9-fold higher in whole washed, inner, and outer mitochondrial fractions, respectively, and 21.6-, 10.2-, and 302-fold higher in nuclear, lysosomal, and microsomal fractions, respectively (supplemented versus deficient rats) | Buttriss and Diplock (1988b) |
Mitochondria | 10, 110, and 1,320 IU/vitamin E (RRR-α-tocopherol-equivalent/kg diet) and coenzyme Q | App. 1.8- and 2.9-fold increase in mitochondria after 14 and 28 days of dietary treatment with 1,320 IU/kg diet, respectively. Treatment with 110 IU/kg resulted in app. 0.8- and 0.9-fold increase after 14 and 28 days of dietary treatment | Ibrahim et al. (2000) |
Mitochondria, microsomes | Daily injections for 9 days with α-tocopherol (subcutaneous) with 10 mg RRR-α-tocopherol/100 g body wt | Total α-tocopherol levels increased 30- and 29-fold in mitochondria and microsomes, respectively (treated vs. control rats) | Gumpricht et al. (2004) |
Mitochondria, microsomes, peroxisomes | 3 daily α-tocopherol injections (subcutaneous) with 10 mg RRR-α-tocopherol/100 g body wt | Total α-tocopherol levels increased 28-fold in microsomes, and 8-fold and 3-fold in mitochondria and peroxisomes, respectively (treated versus control rats) | Mustacich et al. (2010) |