Fig. 7
From: Conjugated linoleic acid isomers modulate protein expression profile in rat hepatocytes
Western blotting of hepatocytes lysates treated with CLA isomers and oleic acid. For each protein line, the following samples have been analyzed: control hepatocytes (line 1); CLA mix-treated hepatocytes (line 2); c9,t11 CLA-treated hepatocytes (line 3); t10,c12 CLA-treated hepatocytes (line 4); oleic acid-treated hepatocytes (line 5). Equal amounts of cell lysates were subjected to SDS-PAGE and probed with anti-FTL1 (a) and anti-PRDX3 (b) specific antibodies. Expression of β-Actin was used to normalize protein loading (c). Protein sizes are reported in the left side of each panel. Densitometric analysis of band intensity is reported for PRDX3 (d) and FTL1 (e), where optical density (OD) values have been used as arbitrary unit