Table 1 Proteins differentially expressed separated by 2DE electrophoresis and identified by mass spectrometry analysis
From: Conjugated linoleic acid isomers modulate protein expression profile in rat hepatocytes
Spot # | Protein description | Gene | Sequence coverage (%) | Mw (kDa) | pI | Score | Swiss-Prot access.# |
|---|---|---|---|---|---|---|---|
1 | ATP synthase subunit d, mitochondrial (ATP5H)* | ATP5H | 64 | 18.8 | 6.21 | 689 | P31399 |
2 | Macrophage migration inhibitory factor (MIF)** | MIF | 13 | 12.6 | 7.28 | 89 | P30904 |
3 | D-Dopachrome decarboxylase (DOPD)* | DDT | 65 | 13.2 | 6.09 | 293 | P80254 |
4 | D-Dopachrome decarboxylase (DOPD)** | DDT | 76 | 13.2 | 6.09 | 206 | P80254 |
5 | Histidine triad nucleotide-binding protein 1 (HINT1)** | HINT1 | 32 | 13.9 | 6.36 | 58 | P62959 |
6 | Fatty acid-binding protein, brain (FABP7)* | FABP7 | 40 | 15.1 | 5.46 | 53 | P55051 |
7 | Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial (IDH3A)** | IDH3A | 13 | 40.0 | 6.47 | 100 | Q9D6R2 |
8 | Thioredoxin-dependent peroxide reductase, mitochondrial (PRDX3)* | PRDX3 | 21 | 28.6 | 7.14 | 323 | Q9Z0V6 |
9 | Agmatinase, mitochondrial (SPEB)* | AGMAT | 12 | 38.4 | 6.71 | 198 | Q0D2L3 |
10 | 6-phosphogluconolactonase (6PGL)* | PGLS | 12 | 27.4 | 5.54 | 40 | P85971 |
11 | Agmatinase, mitochondrial (SPEB)* | AGMAT | 9 | 38.4 | 5.60 | 53 | Q0D2L3 |
12 | Peroxiredoxin-5, mitochondrial (PRDX5)** | PRDX5 | 24 | 22.5 | 8.94 | 91 | Q9R063 |
13 | Ferritin light chain 1 (FTL1)* | FTL1 | 39 | 20.8 | 5.99 | 187 | P02793 |
14 | Isoamyl acetate-hydrolyzing esterase 1 homolog (IAH1)* | IAH1 | 30 | 28.4 | 5.63 | 52 | Q711G3 |
15 | Proteasome subunit beta type-7 (PSB7)* | PSMB7 | 11 | 30.2 | 8.13 | 32 | Q9JHW0 |
16 | Dihydrofolate reductase (DYR)** | DHFR | 29 | 21.7 | 6.77 | 58 | Q920D2 |
17 | ATP synthase subunit d, mitochondrial (ATP5H)** | ATP5H | 49 | 18.8 | 6.17 | 177 | P31399 |
18 | Regucalcin (RGN)* | RGN | 51 | 33.9 | 5.27 | 792 | Q03336 |
19 | Serum paraoxonase/arylesterase 1 (PON1)** | PON1 | 25 | 39.7 | 5.12 | 101 | P55159 |
20 | 3-Hydroxyisobutyrate dehydrogenase, mitochondrial (3HIDH)** | HIBAH | 30 | 35.7 | 8.73 | 454 | P29266 |
21 | Fumarylacetoacetate hydrolase domain-containing protein 1 (FAHD1)** | FAHD1 | 26 | 24.7 | 7.62 | 422 | Q6AYQ8 |
22 | Enoyl-CoA hydratase, mitochondrial (ECHS1)** | ECHS1 | 42 | 31.9 | 8.39 | 779 | P14604 |
23 | Sorbitol dehydrogenase (DHSO)* | SORD | 35 | 38.8 | 7.14 | 172 | P27867 |
24 | Methylmalonate-semialdehyde dehydrogenase, mitochondrial (MMSA)* | ALDH6A1 | 43 | 58.2 | 8.47 | 1,602 | Q02253 |
25 | Carbonic anhydrase 3 (CAH3)* | CA3 | 61 | 29.7 | 6.89 | 258 | P14141 |
26 | Hydroxyacyl-CoA dehydrogenase, mitochondrial (HCDH)* | HADH | 18 | 34.5 | 8.83 | 65 | Q9WVK7 |