Skip to main content

Table 1 Selected protein expression in liver and spleen, and spleen non-heme iron (NHI) content following dietary copper or iron deficiency in rats

From: Suppressed hepcidin expression correlates with hypotransferrinemia in copper-deficient rat pups but not dams

 

Experimental group

Dams1

Dams2

Male pups

CuD

CuA

CuD

CuA

FeD

CuD

CuA

Liver levels

GPI-Cp

0.65 ± 0.06*

1.00 ± 0.03

0.64 ± 0.05c

1.00 ± 0.14b

1.52 ± 0.10a

0.40 ± 0.24*

1.00 ± 0.10

TfR1

0.71 ± 0.22

1.00 ± 0.14

0.75 ± 0.13b

1.00 ± 0.10b

4.85 ± 0.18a

0.55 ± 0.02*

1.00 ± 0.05

TfR2

0.80 ± 0.04*

1.00 ± 0.09

0.66 ± 0.09b

1.00 ± 0.11a

0.45 ± 0.07b

0.30 ± 0.05*

1.00 ± 0.12

Fpn

1.06 ± 0.07

1.00 ± 0.15

1.14 ± 0.18a

1.00 ± 0.24a

0.58 ± 0.05a

1.74 ± 0.18*

1.00 ± 0.12

Spleen levels

GPI-Cp

0.23 ± 0.01*

1.00 ± 0.06

0.12 ± 0.03c

1.00 ± 0.04a

0.78 ± 0.07b

0.51 ± 0.01*

1.00 ± 0.09

TfR1

6.40 ± 0.71*

1.00 ± 0.23

1.44 ± 0.49b

1.00 ± 0.16b

4.04 ± 0.22a

1.94 ± 0.06*

1.00 ± 0.09

Fpn

0.76 ± 0.15

1.00 ± 0.10

0.70 ± 0.05b

1.00 ± 0.13b

1.54 ± 0.16a

1.09 ± 0.11

1.00 ± 0.09

CCS

7.35 ± 0.65*

1.00 ± 0.32

2.61 ± 0.14a

1.00 ± 0.07b

1.41 ± 0.14b

2.88 ± 0.37*

1.00 ± 0.06

NHI, μg/g

462 ± 80.7*

656 ± 33.5

204 ± 39.3b

497 ± 102a

59.8 ± 3.89b

25.0 ± 4.6*

36.2 ± 1.1

  1. Protein expression levels relative to copper-adequate (CuA) rats were determined from densitometric analyses of Western blot data for copper-deficient (CuD) and iron-deficient (FeD) rats (Figs. 3, 4). Means ± SEM (n = 4) are shown, * P < 0.05 compared to CuA. Dams 2 data were analyzed by ANOVA, means with differing superscripts are different, P < 0.05