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Table 1 Selected protein expression in liver and spleen, and spleen non-heme iron (NHI) content following dietary copper or iron deficiency in rats

From: Suppressed hepcidin expression correlates with hypotransferrinemia in copper-deficient rat pups but not dams

  Experimental group
Dams1 Dams2 Male pups
CuD CuA CuD CuA FeD CuD CuA
Liver levels
GPI-Cp 0.65 ± 0.06* 1.00 ± 0.03 0.64 ± 0.05c 1.00 ± 0.14b 1.52 ± 0.10a 0.40 ± 0.24* 1.00 ± 0.10
TfR1 0.71 ± 0.22 1.00 ± 0.14 0.75 ± 0.13b 1.00 ± 0.10b 4.85 ± 0.18a 0.55 ± 0.02* 1.00 ± 0.05
TfR2 0.80 ± 0.04* 1.00 ± 0.09 0.66 ± 0.09b 1.00 ± 0.11a 0.45 ± 0.07b 0.30 ± 0.05* 1.00 ± 0.12
Fpn 1.06 ± 0.07 1.00 ± 0.15 1.14 ± 0.18a 1.00 ± 0.24a 0.58 ± 0.05a 1.74 ± 0.18* 1.00 ± 0.12
Spleen levels
GPI-Cp 0.23 ± 0.01* 1.00 ± 0.06 0.12 ± 0.03c 1.00 ± 0.04a 0.78 ± 0.07b 0.51 ± 0.01* 1.00 ± 0.09
TfR1 6.40 ± 0.71* 1.00 ± 0.23 1.44 ± 0.49b 1.00 ± 0.16b 4.04 ± 0.22a 1.94 ± 0.06* 1.00 ± 0.09
Fpn 0.76 ± 0.15 1.00 ± 0.10 0.70 ± 0.05b 1.00 ± 0.13b 1.54 ± 0.16a 1.09 ± 0.11 1.00 ± 0.09
CCS 7.35 ± 0.65* 1.00 ± 0.32 2.61 ± 0.14a 1.00 ± 0.07b 1.41 ± 0.14b 2.88 ± 0.37* 1.00 ± 0.06
NHI, μg/g 462 ± 80.7* 656 ± 33.5 204 ± 39.3b 497 ± 102a 59.8 ± 3.89b 25.0 ± 4.6* 36.2 ± 1.1
  1. Protein expression levels relative to copper-adequate (CuA) rats were determined from densitometric analyses of Western blot data for copper-deficient (CuD) and iron-deficient (FeD) rats (Figs. 3, 4). Means ± SEM (n = 4) are shown, * P < 0.05 compared to CuA. Dams 2 data were analyzed by ANOVA, means with differing superscripts are different, P < 0.05