Table 2 Primers used in qRT-PCR
Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
β actin; ActB | CAGCCTTCCTTCCTGGGTATG | TAGAGCCACCAATCCACACAG |
Cholesterol 7α-hydroxylase; Cyp7a1 | ACACGCTCTCCACCTTTG | GCTTTCATTGCTTCAGGACTC |
Deiodinase, iodothyronine, type I; Dio1 | GTGATACAGGAAGGCAGGATC | CCTAGAACTGAGGCATGTGTC |
Deiodinase, iodothyronine, type II; Dio2 | CTCCTAGACGCCTACAAACAG | TGCTTCAGGATTGGACAC G |
Fatty acid synthase; Fasn | AATTGCCCGAGTCAGAGAAC | ACAGATCCTTCAGCTTTCCAG |
Glucose-6-phosphate dehydrogenase; G6pd | AGAGGTGGAAACTGACAACG | GCAAAGGTAGCAGTGGTAGAC |
3-hydroxy-3-methyl-glutaryl-CoA reductase; Hmgcr | GCCTCGACCTAATGAAGAGTG | AGTTTGTAGGCTGGGATGTG |
Liver X receptor alpha; Nr1h3 | AGTGCCTGATGTTTCTCCTG | AACCCTATCCTTAAAGCACCC |
Malic enzyme 1, NADP(+)-dependent, cytosolic; Me1 | CAACTCCTATGTGTTCCCTGG | TGACACTTGCTGGGATATGAC |
Phosphogluconate dehydrogenase; PGD | TTGCTCGGTGCTTGTCTTCTCTGA | TGGAAGCATAAAGGGCCTTACGGA |
Cyclophilin A; Ppia | AAGCATACAGGTCCTGGCATCT | TGCCATCCAGCCACTCAGT |
Sterol regulatory element binding transcription factor 1; Srebf1 (Srebp-1c) | CACAGCAACCAGAAACTCAAG | AGCGTTTCTACCACTTCAGG |
Sterol regulatory element binding protein-2; Srebf2 (Srebp2) | CTCTCCTTTAACCCCTTGACTTC | TCAAACCAGCCTCCAGAAC |
Thyroid hormone receptor α; Thra | CAAGGTGGAGTGTGGGTCAGA | CCCTGACATGCTGCTTTTCAG |
Thyroid hormone receptor beta; Thrb | GAGTGGTGGATTTCGCCAAA | GAGGGACATGATCTCCATGCA |
TATA box binding protein; Tbp | CACCAATGACTCCTATGACCC | CAAGTTTACAGCCAAGATTCACG |
18S Ribosomal RNA | ATTCGAACGTCTGCCCTATCAA | CGGGAGTGGGTAATTTGC |