Fig. 2

microRNAs are regulated during TGF-β-stimulated IEC-6 cell differentiation. a Microarray analysis identified 19 miRNAs that significantly correlated with IEC-6 cell differentiation upon TGF-β treatment. Hierarchical clustering analysis was performed using Euclidian distance. Each row represents the relative levels of expression for a significantly regulated single microRNA, and each column represents the relative expression level of biological replicate relative to the untreated control (P < 0.01). Red color of treatment group represents upregulation versus control, and green color of treatment group represents downregulation; b The MA plot shows averaged, background-subtracted fold change (Y axis) and average expression intensity (X axis) of each probe, on both channels for Cy-3-labeled control and Cy-5-labeled treated cells and their dye-swaps. Each dot (insignificantly regulated) or triangle (significantly regulated) represents one miRNA probe. Arrow indicates the miR-146b probe