Fig. 3

hZip1 was partially colocalised to the ER in non-differentiated Caco-2 cells. hZip1 protein showed cytoplasmic granular (gr) and perinuclear staining in some cells (pr) (a, e, i and m). E-cadherin showed a punctate cell junction pattern (b) with no colocalisation between hZip1 and E-cadherin (d). Transferrin receptor was detected as a punctate cytoplasmic pattern (f). The overlay showed no colocalisation between hZip1 and transferrin receptor (h). trans-Golgi marker p230 demonstrated perinuclear label (j) with no colocalisation with hZip1 (l). PDI protein localisation was similar to hZip1 (n) with partial colocalisation (yellow colour) between these proteins was identified in perinuclear region (pr) and in granules located close to this region (pg). Cell nuclei were stained with ethidium bromide (c, g, k and o)