Fig. 4

hZip1 protein was not localised to the cell junction in differentiated Caco-2 cells. Permeabilised, differentiated Caco-2 cells were stained with hZip1 antibody and various cellular markers. Focal plane of the cells (a, b and c). hZip1 at the junctional region between cells (d) but more dispersed than E-cadherin at cell junctions (g). Partial colocalisation between hZIP1 and E-cadherin when overlayed (j). XZ section showing proteins present in different regions (j’). hZip1 showed a diffuse, uniform pattern at the apical surface (e). F-actin was found at cell junctions (h). hZIP1 and F-actin showed no colocalisation (k and k’). Villin highlighted the apical microvilli (i). In the same region, hZip1 (f) demonstrated a similar pattern (l) but the XZ section clearly identified villin in the region above hZip1 (l’)