Fig. 6

Zinc accumulation was increased in Caco-2 cells overexpressing the hZip1 construct and decreased in cells transfected with hZip1 siRNA. a hZip1 mRNA expression levels in Caco-2 cells transfected with hZip1 constructs were higher relative to vector. b Zinc accumulation was increased in Caco-2 cells transfected with hZip1 construct relative to vector-only following incubation in ⁶⁵Zn. c hZip1 mRNA expression levels in Caco-2 cells transfected with hZip1 siRNA was reduced relative to control siRNA. d Zinc accumulation in Caco-2 cells transfected with hZip1 siRNA was reduced compared to control siRNA following incubation in ⁶⁵Zn. The bars represent the mean (±SD) of 3 independent experiments. Stars indicate the statistically significant difference (p < 0.05, t test)