The nutrigenomic investigation of C57BL/6N mice fed a short-term high-fat diet highlights early changes in clock genes expression

Table 6 Relative expression ratio of the eight genes validated by real-time PCR in mice at T2 in response to the HF diet

Gene name	Cecum			Liver
Gene name	Fold change^a (HF/CTR)	SE range	P value	Fold change^a (HF/CTR)	SE range	P value
Aryl hydrocarbon receptor nuclear translocator–like (Arntl) or brain muscle Arnt-like-1 (Bmal1)	↓↓ (0.303)	0.202–0.584	0.002	↓↓↓ (0.053)	0.026–0.089	<0.001
Thyrotroph embryonic factor (Tef)	↑↑↑ (4.926)	3.538–7.028	<0.001	↑↑ (11.146)	6.505–18.958	0.002
Hepatic leukemia factor (Hlf)	↑↑ (4.533)	3.228–9.696	0.001	↑↑ (2.920)	2.048–4.620	0.003
Nuclear receptor subfamily 1, group D, member 2 (Nr1d2) or Rev-erbß	↑↑ (3.112)	2.073–5.300	0.001	↑↑ (4.666)	2.284–8.304	0.003
Nuclear factor, interleukin 3, regulated (Nfil3) or E4 promoter-binding protein 4 (E4BP4)	↓↓↓ (0.352)	0.167–0.674	<0.001	↓↓↓ (0.180)	0.112–0.294	<0.001
Circadian locomotor output cycles kaput (Clock)	–^b	–	0.343	↓↓↓ (0.401)	0.292–0.546	<0.001
Cryptochrome 1 (photolyase-like) (Cry1)	–^b	–	0.890	–^b	–	0.185
Peroxisome proliferator activated receptor alpha (PPARα)	–^b	–	0.73	↑ (1.620)	1.007–2.322	0.021

Data were statistically analyzed by the software REST 2008 (relative expression software tool; [26]; http://rest.gene-quantification.info/)
^aValues are means, n = 6. Arrows indicate the direction and significance level of the difference from control: 1 arrow, 0.01 ≤ P < 0.05; 2 arrows, 0.001 ≤ P < 0.01; 3 arrows, P < 0.001. Fold changes indicate the gene expression difference in mice at T2 induced by the high-fat diet relative to the control diet
^bNot different from the CTR, P > 0.05

ISSN: 1865-3499