Identification of early transcriptome-based biomarkers related to lipid metabolism in peripheral blood mononuclear cells of rats nutritionally programmed for improved metabolic health

Table 1 Nucleotide sequences of primers and amplicon size used for RT-qPCR analysis of mRNA expression levels of selected genes in PBMCs, iWAT, rWAT and liver samples

Gene	Forward primer (5′–3′)	Reverse primer (5′–3′)	Amplicon (bp)
β-actin	TACAGCTTCACCACCACAGC	TCTCCAGGGAGGAAGAGGAT	120
Apob48r	GGGCTACATCAGGCTTTGAG	TTCCTCCCCTACAACCTTCC	150
Cpt1a	GCTCGCACATTACAAGGACAT	TGGACACCACATAGAGGCAG	250
Fasn	CGGCGAGTCTATGCCACTAT	ACACAGGGACCGAGTAAT	222
Gdi-1	CCGCACAAGGCAAATACATC	GACTCTCTGAACCGTCATCAA	210
Lipe	TCACGCTACATAAAGGCTGCT	CCACCCGTAAAGAGGGAACT	169
Lrp1	GAGCAGGTTGTCAGTCAGCA	TAGGGTTTCCGATTTCCACA	187
Pcyt2	CCGACAGGGATGGGTCTG	TGGCTTCCTTCTTCTGATTCC	156
Rxrb	CCCTTCCCAGTCATCAGTTC	GGTGGCTTCACATCTTCAGG	152
Sorl1	CACCGTCTCATTGTCAGCAC	ATCTCGTAGCCCCTGGTTTC	123
Star	GGGTGGATGGGTCAGGTC	CTGCTGGCTTTCCTTCTTCC	168
Tbp	ACCCTTCACCAATGACTCCTATG	ATGATGACTGCAGCAAATCGC	190

Apob48r apolipoprotein B48 receptor, Cpt1a carnitine palmitoyltransferase 1alpha, Fasn fatty acid synthase, Gdi-1 GDP dissociation inhibitor 1, Lipe hormone-sensitive lipase, Lrp1 low density lipoprotein receptor-related protein 1, Pcyt2 phosphate cytidylyltransferase 2, ethanolamine, Rxrb retinoid X receptor beta, Sorl1 sortilin-related receptor, LDLR class A repeats-containing, Star steroidogenic acute regulatory protein and Tbp TATA-box binding protein

ISSN: 1865-3499