Table 3 Microarray data of genes involved in lipid metabolism process and their validation by RT-qPCR in PBMCs samples of male and female offspring of control and calorie-restricted dams during lactation (CR) at the age of 21 days
Related process | Gene name | Gene symbol | Sequence ID | Microarray | qPCR | ||||
|---|---|---|---|---|---|---|---|---|---|
p-value* | Fold change | p-value** | Fold change | ||||||
Males | Females | Males | Females | ||||||
Low density lipoproteins uptake | Apolipoprotein B48 receptor | Apob48r | NM_001109154 | 0.007 | −1.14 | −1.29 | 0.667 | +1.00 | +1.03 |
β-oxidation | Carnitine palmitoyltransferase 1 alpha, liver | Cpt1a | NM_031559 | 0.004 | +1.22 | +1.15 | 0.002 | +1.17 | +1.13 |
Lipogenesis | Fatty acid synthase | Fasn | NM_017332 | 0.002 | −1.11 | −1.25 | 0.025 | −1.19 | −1.03 |
Lipolysis | Lipase, hormone sensitive | Lipe | NM_012859 | 0.010 | +1.14 | +1.1 | 0.004 | +1.09 | +1.07 |
Low density lipoproteins uptake | Low density lipoprotein receptor-related protein 1 | Lrp1 | NM_001130490 | 0.007 | −1.15 | −1.04 | 0.038 | −1.05 | −1.09 |
Glycerophospholipid biosynthesis | Phosphate cytidylyltransferase 2, ethanolamine | Pcyt2 | NM_053568 | 0.000 | −1.07 | −1.24 | 0.166 | −1.00 | −1.10 |
Lipogenesis/lipolysis | Retinoid X receptor beta | Rxrb | NM_206849 | 0.002 | −1.33 | −1.17 | 0.026 | −1.10 | −1.05 |
Low density lipoproteins uptake | Sortilin-related receptor, LDLR class A repeats-containing | Sorl1 | NM_053519 | 0.002 | −1.08 | −1.34 | 0.238 | −1.07 | −1.03 |
Cholesterol transport and uptake | Steroidogenic acute regulatory protein | Star | NM_031558 | 0.006 | +1.11 | +1.09 | 0.047 | +1.09 | +1.10 |