Dietary supplementation with soy isoflavones or replacement with soy proteins prevents hepatic lipid droplet accumulation and alters expression of genes involved in lipid metabolism in rats

Table 1 Composition of experimental diets

Ingredient (g/kg diet)	D1	D2	D3	D4	D5
Casein¹	222.2	222.2	166.5	111.1	–
Soy protein²	–	–	55.6	111.1	222.2
Sucrose	100.0	100.0	100.0	100.0	100.0
Cornstarch	375.3	375.1	375.3	375.3	375.3
Dextronized cornstarch	132.0	132.0	132.0	132.0	132.0
Soybean oil	70.0	70.0	70.0	70.0	70.0
Cellulose	50.0	50.0	50.0	50.0	50.0
Mineral mix³	35.0	35.0	35.0	35.0	35.0
Vitamin mix³	10.0	10.0	10.0	10.0	10.0
Choline Bitartrate	2.5	2.5	2.5	2.52.5
l-Methionine	3.0	3.0	3.0	3.0	3.0
Tert-Butylhydroquinone	0.014	0.014	0.014	0.014	0.014
Novasoy²	–	0.167	–	–	–
Actual content of isoflavones⁴ (mg/kg diet)	0.0	50.1	12.7	25.4	50.1

¹Casein from Harlan Laboratories (Madison, WI) contains 90 % crude protein
²Alcohol-washed soy protein isolate containing 90 % crude protein and Novasoy isoflavone concentrate containing 30 % total isoflavones were purchased from Archer Daniels Midland Company (Decatur, IL)
³AIN-93G Mineral mix (Reeves et al. 1993) and AIN-93G Vitamin mix (Reeves et al. 1993) were from Harlan Laboratories
⁴The actual content of isoflavones was determined by Waters HPLC linear gradient with UV detection monitored at 254 nm (Wang and Murphy 1994)

ISSN: 1865-3499