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Fig. 1 | Genes & Nutrition

Fig. 1

From: Effects of arachidonic acid on the concentration of hydroxyeicosatetraenoic acids in culture media of mesenchymal stromal cells differentiating into adipocytes or osteoblasts

Fig. 1

Adipogenic differentiation. Adipogenic markers of the MSC induced to differentiate into adipocytes in adipogenic medium alone (AM), or supplemented with either 20 μM of arachidonic acid (AA), 20 μM of AA plus 20 μM of DHA (AA + DHA) or 20 μM of AA plus 20 μM of EPA (AA + EPA). a Expression of the pparγ2, lpl and gpd1 adipogenic genes at 7 and 21 days after adipogenic differentiation. The values are relative to the AM control (value of 1). b Optical micrographs of crystal violet staining of the MSC induced to differentiate into adipocytes in the presence or absence of PUFA at day 21. c Quantification of the fat storage by the oil red staining and elution for the different treatments at day 21 of adipogenic induction. The values are relative to the AM control (value of 1). *p < 0.05 in relation to AM

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