Table 1 PCR primer sequences
Gene category | Gene name | Primer sequence (5′ → 3′) | Product size (bp) |
|---|---|---|---|
Adipogenic | Peroxisome proliferator-activated receptor gamma 2 (ppar-γ2) | GCGATTCCTTCACTGATACACTG GAGTGGGAGTGGTCTTCCATTAC | 136 |
Lipoprotein lipase (lpl) | AAGAAGCAGCAAAATGTACCTGAAG CCTGATTGGTATGGGTTTCACTC | 113 | |
Glycerol-3-phosphate dehydrogenase 1 (gpd1) | ATACAGCATCCTCCAGCACAAG GGATGATTCTGCAGGCAGTG | 120 | |
Osteogenic | Runt-related transcription factor 2 (runx2) | TGGTTAATCTCCGCAGGTCAC ACTGTGCTGAAGAGGCTGTTTG | 143 |
Alkaline phosphatase (alp) | CCAACGTGGCTAAGAATGTCATC TGGGCATTGGTGTTGTACGTC | 175 | |
Osteocalcin (oc) | CCATGAGAGCCCTCACACTCC GGTCAGCCAACTCGTCACAGTC | 258 | |
Alox | Arachidonate 5-lipoxygenase (alox5) | AAGGGCGTGGTGACCATTGAG CGAGGTTCTTGCGGAATCGG | 190 |
Arachidonate 12-lipoxygenase (alox12) | TCTCTATGCCCATGATGCTTTACG GGTGAGGAAATGGCAGAGTTGAC | 217 | |
Arachidonate 15-lipoxygenase (alox15) | ACACTTGATGGCTGAGGTCATTG GGTCGAAAATTCCCATGTCAGAG | 155 | |
Arachidonate 15-lipoxygenase, type B (alox15b) | CAAGGGGCTCTGGAGGAGTC AGGAACTGGGAGGCGAAGAAG | 120 | |
Housekeeping | Polymerase RNA II polypeptide A (polr2a) | TTTTGGTGACGACTTGAACTGC CCATCTTGTCCACCACCTCTTC | 125 |