Effects of arachidonic acid on the concentration of hydroxyeicosatetraenoic acids in culture media of mesenchymal stromal cells differentiating into adipocytes or osteoblasts

Table 2 Optimization of MS/MS step for qualitative and quantitative determination of the HETE

Analyte	Precursor ion (m/z)	Voltage MS1 (eV)	Product ion (m/z)	Collision energy (eV)	Quantitation transition (m/z)
15-HETE	319.2	80	175.2	10	319.2 → 175.2
12-HETE	319.2	80	179.2	10	319.2 → 179.2
11-HETE	319.2	80	167.2	15	319.2 → 167.2
8-HETE	319.2	80	155.2	10	319.2 → 155.2
5-HETE	319.2	80	115.2	15	319.2 → 115.2

ISSN: 1865-3499