Table 2 Comparison of genotype and allele frequencies of MTHFR C677T, MTHFR A1298C, SLC19A1 G80A, SLC19A1 C696T, MTRR A66G polymorphisms between REPL patients and control subjects stratified by folic acid supplementation status
Genotype and allele | Folic acid supplementation | |||||||
|---|---|---|---|---|---|---|---|---|
Yes | No | |||||||
Control (n = 74) | Case (n = 54) | AOR (95 % CI) | P value | Control (n = 92) | Case (n = 28) | AOR (95 % CI) | P value | |
MTHFR C677T | ||||||||
 CC | 20 (0.270) | 29 (0.354) | 1.00 (ref) | – | 33 (0.359) | 12 (0.429) | 1.00 (ref) | – |
 CT | 40 (0.541) | 31 (0.574) | 0.90 (0.37–2.11) | 0.812 | 43 (0.467) | 12 (0.439) | 0.75 (0.30–1.89) | 0.540 |
 TT | 14 (0.189) | 6 (0.111) | 0.42 (0.12–1.42) | 0.162 | 16 (0.174) | 4 (0.142) | 0.67 (0.18–2.41) | 0.535 |
 CT+TT |  |  | 0.77 (0.34–1.74) | 0.525 |  |  | 0.73 (0.31–1.73) | 0.469 |
 C | 80 (0.541) | 65 (0.602) | 1.00 (ref) | – | 109 (0.592) | 36 (0.643) | 1.00 (ref) | – |
 T | 68 (0.459) | 43 (0.398) | 0.73 (0.43–1.24) | 0.238 | 75 (0.408) | 20 (0.357) | 0.79 (0.43–1.48) | 0.466 |
MTHFR A1298C | ||||||||
 AA | 62 (0.838) | 31 (0.574) | 1.00 (ref) | – | 70 (0.761) | 18 (0.643) | 1.00 (ref) | – |
 AC | 12 (0.162) | 22 (0.407) | 3.08 (1.30–7.32) | 0.011# | 19 (0.207) | 9 (0.321) | 1.88 (0.72–4.86) | 0.196 |
 CC | 0 (0.000) | 1 (0.019) | $ | $ | 3 (0.032) | 1 (0.036) | 1.38 (0.13–14.13) | 0.788 |
 AC+CC |  |  | 3.26 (1.38–7.70) | 0.007 |  |  | 1.81 (0.73–4.52) | 0.204 |
 A | 136 (0.919) | 84 (0.778) | 1.00 (ref) | – | 159 (0.864) | 45 (0.804) | 1.00 (ref) | – |
 C | 12 (0.081) | 24 (0.222) | 2.87 (1.31–6.26) | 0.008 | 25 (0.136) | 11 (0.196) | 1.59 (0.73–3.50) | 0.246 |
SLC19A1 G80A | ||||||||
 GG | 23 (0.311) | 16 (0.296) | 1.00 (ref) | – | 22 (0.239) | 6 (0.214) | 1.00 (ref) | – |
 GA | 41 (0.554) | 24 (0.444) | 1.15 (0.48–2.78) | 0.755 | 53 (0.576) | 13 (0.464) | 0.88 (0.29–2.61) | 0.813 |
 AA | 10 (0.135) | 14 (0.259) | 2.74 (0.92–8.18) | 0.071 | 17 (0.185) | 9 (0.322) | 1.87 (0.55–6.33) | 0.313 |
 GA+AA |  |  | 1.48 (0.65–3.41) | 0.353 |  |  | 1.12 (0.40–3.12) | 0.833 |
 G | 87 (0.588) | 56 (0.519) | 1.00 (ref) | – | 97 (0.527) | 25 (0.446) | 1.00 (ref) | – |
 A | 61 (0.412) | 52 (0.481) | 1.60 (0.94–2.74) | 0.086 | 87 (0.473) | 31 (0.554) | 1.36 (0.74–2.48) | 0.319 |
SLC19A1 C696T | ||||||||
 CC | 23 (0.311) | 17 (0.315) | 1.00 (ref) | – | 22 (0.239) | 6 (0.214) | 1.00 (ref) | – |
 CT | 40 (0.541) | 23 (0.426) | 1.03 (0.43–2.45) | 0.955 | 54 (0.587) | 12 (0.429) | 0.79 (0.26–2.37) | 0.670 |
 TT | 11 (0.148) | 14 (0.259) | 2.24 (0.77–6.48) | 0.138 | 16 (0.174) | 10 (0.357) | 2.24 (0.67–7.46) | 0.190 |
 CT+TT |  |  | 1.31 (0.58–2.95) | 0.523 |  |  | 1.12 (0.40–3.12) | 0.833 |
 C | 86 (0.581) | 57 (0.528) | 1.00 (ref) | – | 98 (0.533) | 24 (0.429) | 1.00 (ref) | – |
 T | 62 (0.419) | 51 (0.472) | 1.47 (0.86–2.50) | 0.161 | 86 (0.467) | 32 (0.571) | 1.50 (0.82–2.75) | 0.187 |
MTRR A66G | ||||||||
 AA | 41 (0.554) | 43 (0.524) | 1.00 (ref) | – | 57 (0.620) | 16 (0.571) | 1.00 (ref) | – |
 AG | 23 (0.301) | 28 (0.341) | 1.19 (0.52–2.74) | 0.686 | 30 (0.326) | 10 (0.357) | 1.14 (0.45–2.84) | 0.785 |
 GG | 10 (0.135) | 11 (0.134) | 1.82 (0.61–5.44) | 0.283 | 5 (0.054) | 2 (0.072) | 1.27 (0.22–7.43) | 0.787 |
 AG+GG |  |  | 1.36 (0.64–2.87) | 0.423 |  |  | 1.16 (0.48–2.77) | 0.746 |
 A | 105 (0.710) | 72 (0.667) | 1.00 (ref) | – | 144 (0.783) | 42 (0.750) | 1.00 (ref) | – |
 G | 43 (0.290) | 36 (0.333) | 1.40 (0.79–2.48) | 0.248 | 40 (0.217) | 14 (0.250) | 1.14 (0.56–2.31) | 0.724 |